Trehalose-producing Operon treYZ from Arthrobacter ramosus S34

Takuo YAMAMOTO; Kazuhiko MARUTA; Hikaru WATANABE; Hiroshi YAMASHITA; Michio KUBOTA; Shigeharu FUKUDA; Masashi KURIMOTO

doi:10.1271/bbb.65.1419

Microbiology & Fermentation Technology

Trehalose-producing Operon treYZ from Arthrobacter ramosus S34

Takuo YAMAMOTO, Kazuhiko MARUTA, Hikaru WATANABE, Hiroshi YAMASHITA, Michio KUBOTA, Shigeharu FUKUDA, Masashi KURIMOTO

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Keywords: trehalose, gene cloning, nucleotide sequence, operon, Arthrobacter

JOURNAL FREE ACCESS

2001 Volume 65 Issue 6 Pages 1419-1423

DOI https://doi.org/10.1271/bbb.65.1419

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Abstract

Arthrobacter ramosus S34, which produces trehalose from maltooligosaccharide, was isolated. A trehalose-producing operon, treYZ, was cloned from the genome. Expression experiments with treY and treZ confirmed that they coded malto-oligosyltrehalose synthase and malto-oligosyltrehalose trehalohydrolase, respectively. The amino acid sequence of TreY from A. ramosus S34 and that from Arthrobacter sp. Q36 did not show high identity, nor did those to TreZ.

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