Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology
Introduction of Bacterial Metabolism into Higher Plants by Polycistronic Transgene Expression
Hideo NAKASHITAYuko ARAIToshiharu SHIKANAIYoshiharu DOIIsamu YAMAGUCHI
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2001 Volume 65 Issue 7 Pages 1688-1691

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Abstract

Multiple-gene transformation is required to improve or change plant metabolisms effectively; but this many-step procedure is time-consuming and costing. We succeeded in the metabolic engineering of tobacco plants by introducing multiple genes as a bacteria-type operon into a plastid genome. The tobacco plastid was transformed with a polycistron consisting of three bacterial genes for the biosynthesis of a biodegradable polyester, polyhydroxybutyrate (PHB). Accumulation of PHB in the leaves of the transgenic tobacco indicated that the introduced genes were polycistronically expressed. This “phyto-fermentation” system can be used in plant production of various chemical commodities and pharmaceuticals.

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© 2001 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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