Purification and Characterization of L-2,3-Butanediol Dehydrogenase of Brevibacterium saccharolyticum C-1012 Expressed in Escherichia coli

Yusuke TAKUSAGAWA; Masato OTAGIRI; Sadaharu UI; Takashi OHTSUKI; Akio MIMURA; Moriya OHKUMA; Toshiaki KUDO

doi:10.1271/bbb.65.1876

Microbiology & Fermentation Technology

Purification and Characterization of L-2,3-Butanediol Dehydrogenase of Brevibacterium saccharolyticum C-1012 Expressed in Escherichia coli

Yusuke TAKUSAGAWA, Masato OTAGIRI, Sadaharu UI, Takashi OHTSUKI, Akio MIMURA, Moriya OHKUMA, Toshiaki KUDO

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Keywords: 2,3-butanediol dehydrogenase, 2,3-butanediol, acetoin, Brevibacterium saccharolyticum C-1012, short-chain dehydrogenase/reductase family

JOURNAL FREE ACCESS

2001 Volume 65 Issue 8 Pages 1876-1878

DOI https://doi.org/10.1271/bbb.65.1876

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Abstract

The L-2,3-butanediol dehydrogenase produced in E. coli JM109/pLBD2-CTC was purified by 5 steps. The molecular mass of this enzyme was estimated at 110 kDa and the subunit was mesured to be 30 kDa. The L-BDH had some differences from the BDHs from other sources in substrate specificity, pI value, pH stability, effects of divalent cations, and organic acids.

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