2001 Volume 65 Issue 9 Pages 2098-2101
Porcine mammary epithelial cells were isolated to culture on collagen gel followed by gel floating treatment to evaluate differentiation under the culture conditions of serum-free medium, supplemented with combinations of insulin, hydrocortisone, and prolactin. After the culture period, the mammary cells attached to the collagen gels were recovered to observe expression of β-casein, β-lactoglobulin, and lactoferrin by reverse transcriptase polymeric chain reaction method. Expression of β-casein was observed in the presence of insulin, hydrocortisone, and prolactin whereas transcription of β-lactoglobulin and lactoferrin occured irrespective of hydrocortisone and prolactin. Immunoblot analysis demonstrated synthesis and secretion of lactoferrin in the fraction of recovered cells and the culture medium.
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