Biochemistry & Molecular Biology Notes
A Variant of Orpinomyces joyonii 1,3-1,4-β-Glucanase with Increased Thermal Stability Obtained by Random Mutagenesis and Screening
2002 年 66 巻 1 号 p. 171-174
詳細
2002 年 66 巻 1 号 p. 171-174
A thermostable variant of an Orpinomyces joyonii β-glucanase was identified by screening a mutant library constructed using error-prone PCR products. The mutant, designated 2011D, had one amino acid substitution (Val replaced Asp-70). 2011D showed similar catalytic efficiency to its wild-type enzyme, LicA. The temperature at which 50% inactivation occurred after heat treatment for 10 min was increased by 14°C for 2011D, in comparison to those of wild-type enzyme.
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