Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Microbiology & Fermentation Technology Regular Papers
Old Yellow Enzyme from Candida macedoniensis Catalyzes the Stereospecific Reduction of the C=C Bond of Ketoisophorone
Michihiko KATAOKAAtsushi KOTAKAAkiko HASEGAWAMasaru WADAAyumi YOSHIZUMIShigeru NAKAMORISakayu SHIMIZU
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2002 Volume 66 Issue 12 Pages 2651-2657

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Abstract

  Microorganisms were screened for ones that reduced 3,5,5-trimethyl-2-cyclohexene-1,4-dione (ketoisophorone; KIP), and several strains were found to produce (6R)-2,2,6-trimethylcyclohexane-1,4-dione (levodione). The enzyme catalyzing the reduction of the C=C bond of KIP to yield (6R)-levodione was isolated from Candida macedoniensis AKU4588. The results of primary structural analysis and its enzymatic properties suggested that the enzyme might be an Old Yellow Enzyme family protein.

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© 2002 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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