Abstract
Deodorizing effects of tea catechins on amines were examined under alkaline conditions to eliminate the neutralization reaction. They showed deodorizing activity on ethylamine, but none on dimethylamine or trimethylamine. Deodorizing activity on ethylamine was found to be in the order of (−)-epigallocatechin gallate > gallic acid > (−)-epigallocatechin (EGC) > (−)-epicatechin gallate >ethyl gallate>> (+)-catechin=(−)-epicatechin. Further, reaction products of EGC with methylamine, ethylamine, and ammonia were detected by HPLC, indicating that a deodorizing reaction other than neutralization occurs. From structural analysis of the reaction product with the methylamine isolated as a peracetylated derivative, the product was presumed to be methylamine substituted EGC, in which the hydroxyl group of EGC at the 4´ position is replaced by the methylamino group. The same replacement reaction took place in the case of ethylamine and ammonia.
