Effects of Cys Mutation on Taurocholic Acid Transport by Mouse Ileal and Hepatic Sodium-dependent Bile Acid Transporters

Tohru SAEKI; Toshinori KURODA; Makiko MATSUMOTO; Ryuhei KANAMOTO; Kimikazu IWAMI

doi:10.1271/bbb.66.467

Biochemistry & Molecular Biology Notes

Effects of Cys Mutation on Taurocholic Acid Transport by Mouse Ileal and Hepatic Sodium-dependent Bile Acid Transporters

Tohru SAEKI, Toshinori KURODA, Makiko MATSUMOTO, Ryuhei KANAMOTO, Kimikazu IWAMI

著者情報

キーワード: site-directed mutagenesis, sodium-dependent bile acid transporter, transient expression

ジャーナルフリー

2002 年 66 巻 2 号 p. 467-470

DOI https://doi.org/10.1271/bbb.66.467

詳細

抄録

All cysteines of mouse ileal and hepatic sodium-dependent bile acid transporters (Isbt and Ntcp, respectively) were individually replaced by alanine. Replacement of Cys¹⁰⁶ in Isbt and Cys⁹⁶ in Ntcp, which are located closely in alignment, decreased taurocholate uptake. Although Cys⁵¹ in Isbt is conserved in Ntcp, the replacement spoiled Isbt only. Both similarity and difference in the arrangement of functional sites are suggested.

責任著者(Corresponding author)

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