Isolation and Identification of a New Bifidogenic Growth Stimulator Produced by Propionibacterium freudenreichii ET-3

Kakuhei ISAWA; Kenichi HOJO; Nobuo YODA; Tomonori KAMIYAMA; Seiya MAKINO; Mizue SAITO; Hitomi SUGANO; Chinami MIZOGUCHI; Saori KURAMA; Mika SHIBASAKI; Noriko ENDO; Yoshio SATO

doi:10.1271/bbb.66.679

Microbiology & Fermentation Technology Notes

Isolation and Identification of a New Bifidogenic Growth Stimulator Produced by Propionibacterium freudenreichii ET-3

Kakuhei ISAWA, Kenichi HOJO, Nobuo YODA, Tomonori KAMIYAMA, Seiya MAKINO, Mizue SAITO, Hitomi SUGANO, Chinami MIZOGUCHI, Saori KURAMA, Mika SHIBASAKI, Noriko ENDO, Yoshio SATO

Author information

Kakuhei ISAWA
Research & Development Center, Meiji Dairies Corporation
Kenichi HOJO
Food Functionality Research Institute, Meiji Dairies Corporation
Nobuo YODA
Food Functionality Research Institute, Meiji Dairies Corporation
Tomonori KAMIYAMA
Food Functionality Research Institute, Meiji Dairies Corporation
Seiya MAKINO
Food Functionality Research Institute, Meiji Dairies Corporation
Mizue SAITO
Research & Development Center, Meiji Dairies Corporation
Hitomi SUGANO
Food Functionality Research Institute, Meiji Dairies Corporation
Chinami MIZOGUCHI
Food Functionality Research Institute, Meiji Dairies Corporation
Saori KURAMA
Food Functionality Research Institute, Meiji Dairies Corporation
Mika SHIBASAKI
Food Functionality Research Institute, Meiji Dairies Corporation
Noriko ENDO
Food Functionality Research Institute, Meiji Dairies Corporation
Yoshio SATO
Research & Development Center, Meiji Dairies Corporation

Keywords: 1,4-dihydroxy-2-naphthoic acid, bifidogenic growth stimulator, Propionibacterium freudenreichii

JOURNAL FREE ACCESS

2002 Volume 66 Issue 3 Pages 679-681

DOI https://doi.org/10.1271/bbb.66.679

Details

Abstract

We have found a new growth stimulator for bifidobacteria in the culture broth of Propionibacterium freudenreichii ET-3. The bifidogenic growth stimulator (BGS) was purified by Diaion HP-20 column chromatography and preparative HPLC. Spectroscopic methods including ¹H-NMR, UV, and LC-ESI-MS experiments indicated that the chemical structure of the bifidogenic growth stimulator was 1,4-dihydroxy-2-naphthoic acid (DHNA). Approximately 10 mg/L of DHNA was found to be produced in the culture broth of P. freudenreichii ET-3.

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