Characterization of Cloned Mouse Na+/taurocholate Cotransporting Polypeptide by Transient Expression in COS-7 Cells

Tohru SAEKI; Noriki TAKAHASHI; Ryuhei KANAMOTO; Kimikazu IWAMI

doi:10.1271/bbb.66.1116

Biochemistry & Molecular Biology Notes

Characterization of Cloned Mouse Na⁺/taurocholate Cotransporting Polypeptide by Transient Expression in COS-7 Cells

Tohru SAEKI, Noriki TAKAHASHI, Ryuhei KANAMOTO, Kimikazu IWAMI

Author information

Keywords: Na⁺/taurocholate cotransporting polypeptide, transient expression, kinetic analysis, RT-PCR

JOURNAL FREE ACCESS

2002 Volume 66 Issue 5 Pages 1116-1118

DOI https://doi.org/10.1271/bbb.66.1116

Details

Abstract

The mouse Na⁺/taurocholate cotransporting polypeptide transiently expressed in COS-7 cells caused sodium-dependent uptake of [³H]taurocholic acid with K_m and V_max values of 18 μM and 102 pmol/mg protein/min, respectively. This K_m value is comparable to that for rat NTCP and higher than that for human NTCP. Substrate specificity was evaluated by measuring inhibitory effects of unlabeled bile acids on [³H]taurocholic acid transport.

Corresponding author

Register with J-STAGE for free!