Extracellular Acidic Polysaccharide Production by a Two-membered Bacterial Coculture

Shinya KURATA; Kazutaka YAMADA; Kyoko TAKATSU; Satoshi HANADA; Osamu KOYAMA; Toyokazu YOKOMAKU; Yoichi KAMAGATA; Takahiro KANAGAWA; Ryuichiro KURANE

doi:10.1271/bbb.67.8

Microbiology & Fermentation Technology Regular Papers

Extracellular Acidic Polysaccharide Production by a Two-membered Bacterial Coculture

Shinya KURATA, Kazutaka YAMADA, Kyoko TAKATSU, Satoshi HANADA, Osamu KOYAMA, Toyokazu YOKOMAKU, Yoichi KAMAGATA, Takahiro KANAGAWA, Ryuichiro KURANE

Author information

Shinya KURATA
Kankyo Engineering Corporation
Department of Biomolecular Engineering, Tokyo Institute of Technology
Kazutaka YAMADA
Kankyo Engineering Corporation
Kyoko TAKATSU
Kankyo Engineering Corporation
Satoshi HANADA
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST)
Osamu KOYAMA
Kankyo Engineering Corporation
Toyokazu YOKOMAKU
Kankyo Engineering Corporation
Yoichi KAMAGATA
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST)
Takahiro KANAGAWA
Department of Biomolecular Engineering, Tokyo Institute of Technology
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST)
Ryuichiro KURANE
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST)

Keywords: coculture, extracellular polysaccharide, succinoglycan, Cellulomonas, Agrobacterium

JOURNAL FREE ACCESS

2003 Volume 67 Issue 1 Pages 8-14

DOI https://doi.org/10.1271/bbb.67.8

Details

Abstract

A two-membered coculture of strains KYM-7 and KYM-8, identified as Cellulomonas cellulans and Agrobacterium tumefaciens, respectively, produced a large amount of an extracellular polysaccharide, designated APK-78, from starch. Each strain in pure culture produced only very little amount of polysaccharide from starch; the coexistence of the two strains from the early stage of cultivation was indispensable for a large amount of polysaccharide to be produced. The polysaccharide APK-78 was acidic and composed of glucose, galactose, succinic acid, and pyruvic acid with a molar ratio of 8.1:1.0:1.7:1.0, indicating that it is a succinoglycan type of polysaccharide.

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