PEG-Inhibitor Conjugates: Application of Diphenyl α-Aminoalkylphosphonate to Removal of Chymotrypsin

Makiko UMEZAKI; Takayoshi FUJII; Toshiaki YOSHIMURA; Isao YAMAZAKI; Shin ONO

doi:10.1271/bbb.67.2273

Biochemistry & Molecular Biology Notes

PEG-Inhibitor Conjugates: Application of Diphenyl α-Aminoalkylphosphonate to Removal of Chymotrypsin

Makiko UMEZAKI, Takayoshi FUJII, Toshiaki YOSHIMURA, Isao YAMAZAKI, Shin ONO

著者情報

キーワード: affinity separation, diphenyl α-aminoalkylphosphonate, irreversible inhibitor, poly(ethylene glycol), serine proteases

ジャーナルフリー

2003 年 67 巻 10 号 p. 2273-2276

DOI https://doi.org/10.1271/bbb.67.2273

詳細

抄録

Diphenyl 1-amino-2-phenylethylphosphonate was introduced to poly(ethylene glycol)s (PEGs) with average molecular masses of 300, 400, and 600 to prepare water-insoluble PEG-inhibitor conjugates. Interestingly, only the conjugate from PEG with an average molecular weight of 600 formed a precipitate with chymotrypsin but not with trypsin. The results demonstrated that the PEG-inhibitor conjugate is useful for separation of chymotrypsin.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）