Addition of a Peptide Tag at the C Terminus of AtHKT1 Inhibits Its Na⁺ Transport

Abstract

  Eight transmembrane segments in the Arabidopsis Na⁺ transporter, AtHKT1, have been proposed to be arranged around the axis of the pore. We report here that the addition of a GFP or a FLAG tag to the C terminus of AtHKT1 impairs the Na⁺ transport in Xenopus ooytes as detected by electrophysiological measurements.