Improvement of Reverse Transcription PCR by RNase H

抄録

  An improvement in the method of the Reverse Transcription PCR (RT-PCR) using RNase H is proposed here. We succeeded in RT-PCR amplification against the full sequence of the coding region (8.9 kb) of the Insulin-like growth factor II receptor gene which has the area called the GC-block of about 90% GC contents at the 5′ terminal. Furthermore, the RNase H treatment improved the sensitivity of RT-PCR amplification against a general target.