Protein Profile of Symbiotic Bacteria Mesorhizobium loti MAFF303099 in Mid-growth Phase

Hideyuki KAJIWARA; Takayo KANEKO; Masami ISHIZAKA; Shigeyuki TAJIMA; Hiroshi KOUCHI

doi:10.1271/bbb.67.2668

Biochemistry & Molecular Biology Communications

Protein Profile of Symbiotic Bacteria Mesorhizobium loti MAFF303099 in Mid-growth Phase

Hideyuki KAJIWARA, Takayo KANEKO, Masami ISHIZAKA, Shigeyuki TAJIMA, Hiroshi KOUCHI

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Keywords: mass spectrometry, Mesorhizobium loti, proteome, two-dimensional polyacrylamide gel electrophoresis

JOURNAL FREE ACCESS

2003 Volume 67 Issue 12 Pages 2668-2673

DOI https://doi.org/10.1271/bbb.67.2668

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Abstract

Expressed proteins in cultured symbiotic bacteria (Mesorhizobium loti MAFF303099) in the mid-growth phase were proteomically analyzed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and capillary high-performance liquid chromatography coupled with an ion-trap mass spectrometry (MS). The genome sequence data of M. loti were used to identify the analyzed proteins. We identified 114 of the 127 proteins analyzed on 2D-PAGE gel with some microheterogenities which were caused by post-translational modifications.

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