High IgM Production by Human-human Hybridoma HB4C5 Cells Cultured in Microtubes

Takuya SUGAHARA; Satomi YANO; Takeshi SASAKI

doi:10.1271/bbb.67.393

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Microbiology & Fermentation Technology Note

High IgM Production by Human-human Hybridoma HB4C5 Cells Cultured in Microtubes

Takuya SUGAHARA, Satomi YANO, Takeshi SASAKI

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Keywords: cell culture vessel, human-human hybridoma, immunoglobulin production, physical stimulation

JOURNAL FREE ACCESS

2003 Volume 67 Issue 2 Pages 393-395

DOI https://doi.org/10.1271/bbb.67.393

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Abstract

HB4C5 cells, a human-human hybridoma, were cultured in microtubes. After 24 h of cultivation, growth of the cells cultured in microtubes was 1.2- to 1.5-fold that in culture dishes or 96-well culture plates. The production of IgM was 2.6- to 3.3-fold that in the 96-well culture plates.

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