Authentic and Recombinant Bilirubin Oxidases Are in Different Resting Forms

Takeshi SAKURAI; Lei ZHAN; Takahiro FUJITA; Kunishige KATAOKA; Atsushi SHIMIZU; Tatsuya SAMEJIMA; Shotaro YAMAGUCHI

doi:10.1271/bbb.67.1157

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Biochemistry & Molecular Biology Notes

Authentic and Recombinant Bilirubin Oxidases Are in Different Resting Forms

Takeshi SAKURAI, Lei ZHAN, Takahiro FUJITA, Kunishige KATAOKA, Atsushi SHIMIZU, Tatsuya SAMEJIMA, Shotaro YAMAGUCHI

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Keywords: bilirubin oxidase, multicopper oxidase, trinuclear Cu center

JOURNAL FREE ACCESS

2003 Volume 67 Issue 5 Pages 1157-1159

DOI https://doi.org/10.1271/bbb.67.1157

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Abstract

Myrothecium verrucaria bilirubin oxidase expressed in Aspergillus oryzae is in a resting form different from that of the authentic bilirubin oxidase, but reaches the resting form of the authentic enzyme after one cycle of reduction and reoxidation with dioxygen as shown by the absorption and electron paramagnetic resonance spectra.

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