Molecular Cloning of Quinohemoprotein Alcohol Dehydrogenase, ADH IIB, from Pseudomonas putida HK5

Hirohide TOYAMA; Takaaki FUJII; Nahoko AOKI; Kazunobu MATSUSHITA; Osao ADACHI

doi:10.1271/bbb.67.1397

Biochemistry & Molecular Biology Notes

Molecular Cloning of Quinohemoprotein Alcohol Dehydrogenase, ADH IIB, from Pseudomonas putida HK5

Hirohide TOYAMA, Takaaki FUJII, Nahoko AOKI, Kazunobu MATSUSHITA, Osao ADACHI

Author information

Keywords: quinoprotein, quinohemoprotein, pyrroloquinoline quinone, PQQ, alcohol dehydrogenase

JOURNAL FREE ACCESS

2003 Volume 67 Issue 6 Pages 1397-1400

DOI https://doi.org/10.1271/bbb.67.1397

Details

Abstract

Molecular cloning of the gene of quinohemoprotein alcohol dehydrogenase (ADH IIB) from Pseudomonas putida HK5 was done. The gene (qbdA) was 690 amino acids in length, containing a 22-amino acid signal sequence. Another gene (qbdB) upstream of qbdA, probably in the same transcriptional unit, was found. Further upstream, a gene divergently transcribed against qbdBA had identity with NAD-dependent aldehyde dehydrogenase.

Corresponding author

Register with J-STAGE for free!