Stimulated Accumulation of Lectin mRNA and Stress Response in Helianthus tuberosus Callus by Methyl Jasmonate

Ryoji NAKAGAWA; Yukihiro OKUMURA; Makoto KAWAKAMI; Daisuke YASOKAWA; Koji NAGASHIMA

doi:10.1271/bbb.67.1822

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Biochemistry & Molecular Biology Notes

Stimulated Accumulation of Lectin mRNA and Stress Response in Helianthus tuberosus Callus by Methyl Jasmonate

Ryoji NAKAGAWA, Yukihiro OKUMURA, Makoto KAWAKAMI, Daisuke YASOKAWA, Koji NAGASHIMA

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Keywords: Helianthus tuberosus, jasmonate, lectin, DNA fragmentation, self-fluorescence

JOURNAL FREE ACCESS

2003 Volume 67 Issue 8 Pages 1822-1824

DOI https://doi.org/10.1271/bbb.67.1822

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Abstract

Callus from Helianthus tuberosus expresses a mannose-specific lectin (HTA). The level of HTA mRNA significantly increased one hour after treatment of the callus with 20 mg/l methyl jasmonate. Following this, fragmentation of the callus DNA at regular intervals was observed together with strong self-fluorescence emission in the callus cells.

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