Purification and Characterization of α-Keto Amide Reductase from Saccharomyces cerevisiae

Kohji ISHIHARA; Hiroaki YAMAMOTO; Kazuya MITSUHASHI; Kazuyoshi NISHIKAWA; Sadao TSUBOI; Hideaki TSUJI; Nobuyoshi NAKAJIMA

doi:10.1271/bbb.68.2306

Microbiology & Fermentation Technology Regular Papers

Purification and Characterization of α-Keto Amide Reductase from Saccharomyces cerevisiae

Kohji ISHIHARA, Hiroaki YAMAMOTO, Kazuya MITSUHASHI, Kazuyoshi NISHIKAWA, Sadao TSUBOI, Hideaki TSUJI, Nobuyoshi NAKAJIMA

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Keywords: Saccharomyces cerevisiae, α-keto amide, α-keto ester, reduction, reductase

JOURNAL FREE ACCESS

2004 Volume 68 Issue 11 Pages 2306-2312

DOI https://doi.org/10.1271/bbb.68.2306

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Abstract

An NADPH-dependent α-keto amide reductase was purified from Saccharomyces cerevisiae. The molecular mass of the native enzyme was estimated to be 33 and 36 kDa by gel filtration chromatography and SDS–polyacrylamide gel electrophoresis, respectively. The purified enzyme showed a reducing activity not only for aromatic α-keto amides but also for aliphatic and aromatic α-keto esters. The internal sequence of the enzyme was identical with that of a hypothetical protein (ORF YDL 124w) coded by yeast chromosome IV.

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