Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
Identification of Peptidyl Mimics of Bioactive Gibberellin Recognized by an Antibody
Haruko SEKIMOTOYoshihito SUZUKIIsomaro YAMAGUCHI
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2004 年 68 巻 11 号 p. 2408-2410

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We screened a phage display peptide library for peptidyl mimotopes of gibberellin against anti-bioactive gibberellin antibody. The peptides obtained were grouped into two homologous sequences and their binding to the antibody was put in competition with free GA4 but not with GA4 methylester, suggesting that the peptides behave as mimics of GA4. As an application, the phage display peptide was shown to work as a tracer for enzyme-linked immunosorbent assay (ELISA) analysis of GA4.
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© 2004 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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