Specification of Amino Acid Residues Essential for the Catalytic Reaction of Cold-active Protein-tyrosine Phosphatase of a Psychrophile, Shewanella sp.

Hiroki TSURUTA; Jun TAMURA; Hiroshi YAMAGATA; Yasuo AIZONO

doi:10.1271/bbb.68.440

Biochemistry & Molecular Biology Notes

Specification of Amino Acid Residues Essential for the Catalytic Reaction of Cold-active Protein-tyrosine Phosphatase of a Psychrophile, Shewanella sp.

Hiroki TSURUTA, Jun TAMURA, Hiroshi YAMAGATA, Yasuo AIZONO

著者情報

キーワード: cold-active enzyme, protein-tyrosine-phosphatase, phosphoesterase motif, site-directed mutagenesis

ジャーナルフリー

2004 年 68 巻 2 号 p. 440-443

DOI https://doi.org/10.1271/bbb.68.440

詳細

抄録

Protein-tyrosine phosphatase [EC 3.1.3.48] from a psychrophile, Shewanella sp. shows high activity at low temperatures and has the conserved amino acid sequence of protein-Ser/Thr-phosphatases. Site-directed mutagenesis with the conserved amino acid residues indicated that His148 could be important as a general acid catalyst and Asp115 assists the protonation with His148 of the leaving group of a substrate, and that Asp76 and Asp112 were involved in binding to magnesium ions.

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