Transcriptional Analysis of the ostA/imp Gene Involved in Organic Solvent Sensitivity in Escherichia coli

Abstract

Transcriptional initiation sites of the ostA gene involved in organic solvent sensitivity in Escherichia coli were found by primer extension analysis. Two transcriptional initiation sites were newly identified at −133 and −48 nucleotides from the initiation codon of ostA, but the previously reported σ^E-dependent one at −227 could not be detected. No heat-inducible expression of ostA was observed by Northern blotting analysis, indicating that the contribution of σ^E-dependent transcription was very small if any. σ^D-Dependent promoter-like sequences were found just upstream of the newly identified transcriptional initiation sites by computer-aided analysis. Deletion analysis of ostA-lacZ fusions demonstrated that these two promoters contributed almost equally to the constitutive expression of the ostA gene.