The Role of the DRY Motif of Human MC4R for Receptor Activation

Yoshiaki YAMANO; Rio KAMON; Takao YOSHIMIZU; Yoshihisa TODA; Yuichi OSHIDA; Shigeyuki CHAKI; Masanobu YOSHIOKA; Isao MORISHIMA

doi:10.1271/bbb.68.1369

Biochemistry & Molecular Biology Notes

The Role of the DRY Motif of Human MC4R for Receptor Activation

Yoshiaki YAMANO, Rio KAMON, Takao YOSHIMIZU, Yoshihisa TODA, Yuichi OSHIDA, Shigeyuki CHAKI, Masanobu YOSHIOKA, Isao MORISHIMA

Author information

Yoshiaki YAMANO
Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University
Rio KAMON
Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University
Takao YOSHIMIZU
Medicinal Pharmacology Laboratory, Taisho Pharmaceutical Co., Ltd.
Yoshihisa TODA
Medicinal Pharmacology Laboratory, Taisho Pharmaceutical Co., Ltd.
Yuichi OSHIDA
Medicinal Pharmacology Laboratory, Taisho Pharmaceutical Co., Ltd.
Shigeyuki CHAKI
Medicinal Pharmacology Laboratory, Taisho Pharmaceutical Co., Ltd.
Masanobu YOSHIOKA
Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University
Isao MORISHIMA
Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University

Keywords: melanocortin type 4 receptor, site directed mutagenesis, cAMP, G-protein coupled receptor, DRY motif

JOURNAL FREE ACCESS

2004 Volume 68 Issue 6 Pages 1369-1371

DOI https://doi.org/10.1271/bbb.68.1369

Details

Abstract

We constructed several mutant human MC4R cDNAs by site directed mutagenesis and expressed these receptors in COS-1 cells. The conserved DRY motif among GPCRs was mutated to generate eight mutants. While no MC4R ligand binding was detected in any of the mutants, one mutant, D146A, resulted in higher cAMP production in cells than the wild-type receptor without ligand stimulation.

Corresponding author

Register with J-STAGE for free!