Mitogenic Properties of Pokeweed Lectin-D Isoforms on Human Peripheral Blood Lymphocytes: Non-mitogen PL-D1 and Mitogen PL-D2

Kenichi YAMAGUCHI; Mizuki UECHI; Yoshinori KATAKURA; Tatsuya ODA; Masatsune ISHIGURO

doi:10.1271/bbb.68.1591

Biochemistry & Molecular Biology Notes

Mitogenic Properties of Pokeweed Lectin-D Isoforms on Human Peripheral Blood Lymphocytes: Non-mitogen PL-D1 and Mitogen PL-D2

Kenichi YAMAGUCHI, Mizuki UECHI, Yoshinori KATAKURA, Tatsuya ODA, Masatsune ISHIGURO

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Keywords: pokeweed mitogen, chitin-binding lectin, hevein domain, Phytolacca americana

JOURNAL FREE ACCESS

2004 Volume 68 Issue 7 Pages 1591-1593

DOI https://doi.org/10.1271/bbb.68.1591

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Abstract

We investigated native structures and mitogenic properties of pokeweed lectin-D isoforms (PL-D1 and -D2) on human peripheral blood lymphocytes along with other isolectins (PL-A to -C). Both native PL-D isoforms appeared to behave as monomers. PL-D2 proliferated the lymphocytes like PL-C, whereas PL-D1 had no mitogenicity. PL-D1 acquired mitogenic activity after trimming of the C-terminal dipeptide.

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