Dihydrocoronatine, Promising Candidate for a Chemical Probe to Study Coronatine-, Jasmonoid- and Octadecanoid-binding Protein

Abstract

Coronatine (1), its synthetic analogs (6-13) and jasmonic acid induced various volatiles in rice leaves. In the range of 0.01–0.1 mM, dihydrocoronatine (7) exhibited 4-687 times higher activity for linalool emission than that of 1. The radioactive derivative of 7, [4,5-³H]-7, was employed to identify the putative coronatine-binding protein in rice leaves. 7 would be a promising candidate for a chemical probe to study cornatine-binding protein related to the jasmonoid and octadecanoid signaling pathway in higher plants. A detailed study of coronatine-binding protein in rice leaves and cell culture with [4,5-³H]-7 is now in progress.