Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Regular Papers
Functional Expression and Characterization of a Bacterial Light-harvesting Membrane Protein in Escherichia coli and Cell-free Synthesis Systems
Yuichiro SHIMADAZheng-Yu WANGYushi MOCHIZUKIMasayuki KOBAYASHITsunenori NOZAWA
Author information
JOURNALS FREE ACCESS

2004 Volume 68 Issue 9 Pages 1942-1948

Details
Abstract

Heterologous expression of a bacterial light-harvesting (LH) integral membrane protein was attempted using Escherichia coli cells and cell-free synthesis systems prepared from E. coli extracts. The α-apoprotein of LH1 complex from purple photosynthetic bacterium Rhodospirillum rubrum was overexpressed as a recombinant protein with a histidine (His6) tag added to the carboxyl terminus. Both of the expression systems produced α-apoprotein in a fully functional form as can judged by its ability to form a structural subunit with native β-apoprotein and the pigment molecule bacteriochlorophyll a. The expression product in E. coli appears to be located in the inner cell membrane and can be almost completely extracted by 0.5% (w/v) Triton X-100. Circular dichroism measurement indicated that the expressed α-apoproteins from both systems had α-helical contents essentially identical with that of the native one. About two thirds of the α-apoprotein expressed in E. coli was found to have the amino terminal methionine residue modified by a formyl group. About one third of the α-apoprotein expressed in the cell-free system was found to be oxidized at the side chain of the amino terminal methionine residue. Functional expression of the α-apoprotein using the cell-free system provides an useful example for producing highly hydrophobic integral membrane proteins with relatively large quantities sufficient for biophysical and structural analysis.

Information related to the author

This article cannot obtain the latest cited-by information.

© 2004 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
Previous article Next article
feedback
Top