Identification of Insertion Sequence from a γ-Hexachlorocyclohexane Degrading Bacterium, Sphingomonas paucimobilis UT26

Keisuke MIYAUCHI; Masao FUKUDA; Masataka TSUDA; Masamichi TAKAGI; Yuji NAGATA

doi:10.1271/bbb.69.216

Biochemistry & Molecular Biology Notes

Identification of Insertion Sequence from a γ-Hexachlorocyclohexane Degrading Bacterium, Sphingomonas paucimobilis UT26

Keisuke MIYAUCHI, Masao FUKUDA, Masataka TSUDA, Masamichi TAKAGI, Yuji NAGATA

Author information

Keywords: biodegradation, insertion sequence, Tn5 mutagenesis, Sphingomonas, γ-hexachlorocyclohexane

JOURNAL FREE ACCESS

2005 Volume 69 Issue 1 Pages 216-219

DOI https://doi.org/10.1271/bbb.69.216

Details

Abstract

Tn5-derived mutants of the γ-hexachlorocyclohexane-degrading bacterium Sphingomonas paucimobilis UT26 were genetically characterized, and an endogenous insertion sequence (IS) which belongs to the IS1380 family was identified. The IS, named ISsp1, existed as multi copies in UT26, and its transposition appeared to be activated during the process of Tn5-mutagenesis. It was found that transposon mutagenesis can cause endogenous mutations.

Corresponding author

Register with J-STAGE for free!