Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University Department of Physics, Graduate School of Science, Kyoto University, and CREST
Noriyuki KUMAZAWA
Faculty of Engineering, Ibaraki University
Kenichi YOSHIKAWA
Department of Physics, Graduate School of Science, Kyoto University, and CREST
Yasurou KURUSU
Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University
Transformation system for Escherichia coli based upon introduction of plasmid DNA by natural phospholipids has been developed. Transformants are easily obtained by treatment with natural phospholipids such as phosphatidylethanolamine, phosphatidylcoline, and phosphatidylserine, where the presence of MgCl2 or CaCl2 is essential. This method of transformation is applicable not only for small plasmid pHSG399 (2.3 Kb) but also for giant plasmid R6K (100 Kb).
