Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Food & Nutrition Science Regular Papers
Prevention of Intestinal Infection by Glycomacropeptide
Kouhei NAKAJIMANorimitsu TAMURAKazuo KOBAYASHI-HATTORITadashi YOSHIDAYukiko HARA-KUDOMasanari IKEDOYoshiko SUGITA-KONISHIMakoto HATTORI
Author information
JOURNALS FREE ACCESS

2005 Volume 69 Issue 12 Pages 2294-2301

Details
Abstract

The preventive effects of glycomacropeptide (GMP) against intestinal infection were investigated, and conjugates of GMP with xylooligosaccharide (XOS) and carboxymethyldextran (CMD) were prepared by the Maillard reaction to enhance the effect of GMP. The binding ability of GMP to intestinal pathogenic bacteria was evaluated by a binding assay with biotinylated bacteria. GMP showed the ability to bind to Salmonella enteritidis and enterohemorrhagic Escherichia coli O157:H7 (EHEC O157). This binding ability was decreased by a sialidase treatment and completely eliminated by periodate oxidation. These results indicate that such carbohydrate moieties as sialic acid in GMP are involved in binding to S. enteritidis and EHEC O157. The preventive effect of GMP on the adhesion of pathogenic bacteria to Caco-2 cells was also investigated. GMP showed an inhibitory effect on the adhesion of EHEC O157 in a dose-dependent manner, although it was not a potent inhibitor of the adhesion of Salmonella infection. However, in the case of Salmonella infection, GMP–XOS and GMP–CMD significantly suppressed IL-8 production which was the index of infection. Our results indicate GMP to be a promising agent for preventing intestinal infection.

Information related to the author

This article cannot obtain the latest cited-by information.

© 2005 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
Previous article Next article
feedback
Top