Cloning, Expression in Yeast, and Functional Characterization of CYP76A4, a Novel Cytochrome P450 of Petunia That Catalyzes (ω-1)-Hydroxylation of Lauric Acid

Katsutomo TAMAKI; Hiromasa IMAISHI; Hideo OHKAWA; Kiyoharu OONO; Mikihiro SUGIMOTO

doi:10.1271/bbb.69.406

Biochemistry & Molecular Biology Notes

Cloning, Expression in Yeast, and Functional Characterization of CYP76A4, a Novel Cytochrome P450 of Petunia That Catalyzes (ω-1)-Hydroxylation of Lauric Acid

Katsutomo TAMAKI, Hiromasa IMAISHI, Hideo OHKAWA, Kiyoharu OONO, Mikihiro SUGIMOTO

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Keywords: cytochrome P450, Saccharomyces cerevisiae, lauric acid, (ω-1)-hydroxylation

JOURNAL FREE ACCESS

2005 Volume 69 Issue 2 Pages 406-409

DOI https://doi.org/10.1271/bbb.69.406

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Abstract

A cDNA clone of a novel cytochrome P450, CYP76A4, was isolated from Petunia hybrida. The cDNA clone contained an open reading frame (ORF) encoding a predicted 510 amino acid polypeptide. The CYP76A4 cDNA was expressed in yeast Saccharomyces cerevisiae AH22. Recombinant yeast microsomes containing the CYP76A4 hemoprotein were found to catalyze (ω-1)-hydroxylation of lauric acid.

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