Involvement of Saccharomyces cerevisiae Pdr5p ATP-Binding Cassette Transporter in Calcium Homeostasis

Andreea Cristina TUTULAN-CUNITA; Tomoko OHNISHI; Masaki MIZUNUMA; Dai HIRATA; Tokichi MIYAKAWA

doi:10.1271/bbb.69.857

Biochemistry & Molecular Biology Notes

Involvement of Saccharomyces cerevisiae Pdr5p ATP-Binding Cassette Transporter in Calcium Homeostasis

Andreea Cristina TUTULAN-CUNITA, Tomoko OHNISHI, Masaki MIZUNUMA, Dai HIRATA, Tokichi MIYAKAWA

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Keywords: Pdr5p, calcium homeostasis, ATP-binding cassette (ABC) transporter, Saccharomyces cerevisiae

JOURNAL FREE ACCESS

2005 Volume 69 Issue 4 Pages 857-860

DOI https://doi.org/10.1271/bbb.69.857

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Abstract

Deletion of PDR5 gene (Δpdr5) in Saccharomyces cerevisiae led to increased resistance to calcium. The cellular Ca²⁺ level in the presence of high calcium as estimated by reporter assay in Δpdr5 cells was significantly lower than that in wild-type cells. Membrane Pdr5p levels diminished rapidly during incubation with high calcium in a manner dependent on calcineurin and Pep4p, suggesting a feedback regulatory mechanism for Pdr5p abundance.

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