Immobilization of Lipase on Epoxy Activated (1→3)-α-<small>D</small>-Glucan Isolated from Penicillium chrysongenum

Tianqi WANG; Hanxiang LI; Kaili NIE; Tianwei TAN

doi:10.1271/bbb.60292

Biochemistry & Molecular Biology Regular Papers

Immobilization of Lipase on Epoxy Activated (1→3)-α-D-Glucan Isolated from Penicillium chrysongenum

Tianqi WANG, Hanxiang LI, Kaili NIE, Tianwei TAN

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Keywords: immobilization, Candida sp. lipase, Penicillium chrysongenum, (1→3)-α-D-glucan, epichlorohydrin

JOURNAL FREE ACCESS

2006 Volume 70 Issue 12 Pages 2883-2888

DOI https://doi.org/10.1271/bbb.60292

View "Advance Publication" version (December 7, 2006).

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Abstract

A water-insoluble linear (1→3)-α-D-glucan was isolated from Penicillium mycelia. Three kinds of epoxy-activated microspheres of this glucan were prepared as supports for Candida sp. lipase (EC3.1.1.3) immobilization. The highest immobilization yield was 36.4%. The specific activity was 26.85 U/mg, and only 4.1% of activity was lost in comparison with the free enzyme used for immobilization. The higher thermal stability, storage stability, and reusability of the immobilized lipase make it a potential candidate for wide application.

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