Conjugational Transfer Kinetics of pLS20 between Bacillus subtilis in Liquid Medium

Mitsuhiro ITAYA; Nagayoshi SAKAYA; Satoko MATSUNAGA; Kyoko FUJITA; Shinya KANEKO

doi:10.1271/bbb.70.740

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Microbiology & Fermentation Technology Notes

Conjugational Transfer Kinetics of pLS20 between Bacillus subtilis in Liquid Medium

Mitsuhiro ITAYA, Nagayoshi SAKAYA, Satoko MATSUNAGA, Kyoko FUJITA, Shinya KANEKO

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Keywords: Bacillus subtilis, conjugational transfer, pLS20

JOURNAL FREE ACCESS

2006 Volume 70 Issue 3 Pages 740-742

DOI https://doi.org/10.1271/bbb.70.740

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Abstract

pLS20-mediated conjugational transfer between Bacillus subtilis was investigated not on conventional solid media but in liquid culture. Detailed conjugational kinetics revealed that pLS20 transmission occurred at a limited cellular growth stage of both donor and recipient. Mutation of the recipient recA did not significantly interfere with the conjugational transfer process.

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