Properties of a Trifluoroleucine-Resistant Mutant of Saccharomyces cerevisiae

Takahiro OBA; Yoshitsugu YAMAMOTO; Shuji NOMIYAMA; Hikaru SUENAGA; Shigeru MUTA; Kosuke TASHIRO; Satoru KUHARA

doi:10.1271/bbb.50640

Microbiology & Fermentation Technology Note

Properties of a Trifluoroleucine-Resistant Mutant of Saccharomyces cerevisiae

Takahiro OBA, Yoshitsugu YAMAMOTO, Shuji NOMIYAMA, Hikaru SUENAGA, Shigeru MUTA, Kosuke TASHIRO, Satoru KUHARA

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Keywords: Saccharomyces cerevisiae, 5,5,5-trifluoro-DL-leucine, leucine feedback inhibition, i-AmOH, DNA microarray analysis

JOURNAL FREE ACCESS

2006 Volume 70 Issue 7 Pages 1776-1779

DOI https://doi.org/10.1271/bbb.50640

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Abstract

We characterized a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae, TFL20, that has a mutation in the LEU4 gene. We monitored the concentration of extracellular i-AmOH and intracellular amino acids, and compared the ratios of gene expression in TFL20 with the wild-type strain, K30. We found that the LEU1, LEU2, and BAT1 genes were up-regulated in TFL20 for metabolism, and that TFL20 simultaneously produced as much i-AmOH and leucine as K30 does.

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