Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
Preparation of N-Acylated Proteins Modified with Fatty Acids Having a Specific Chain Length Using an Insect Cell-Free Protein Synthesis System
Takashi SUZUKIMasaaki ITOToru EZUREMasamitsu SHIKATAEiji ANDOToshihiko UTSUMISusumu TSUNASAWAOsamu NISHIMURA
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2007 Volume 71 Issue 1 Pages 261-264

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Abstract

To establish a strategy to generate N-acylated proteins modified with fatty acids having a specific chain length, tGelsolin-streptag, an epitope-tagged model protein having an N-myristoylation motif, was synthesized using an insect cell-free protein synthesis system in the presence of acyl-CoA with various fatty acid chain lengths. It was found that the fatty acid species attached to the N-termini fully depended on the acyl-CoA species added to the reaction mixture. N-Acylated proteins with fatty acid chain lengths of 8, 10, 12, and 14 were generated successfully.

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© 2007 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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