Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Food & Nutrition Science Regular Papers
Anti-Histone Acetyltransferase Activity from Allspice Extracts Inhibits Androgen Receptor-Dependent Prostate Cancer Cell Growth
Yoo-Hyun LEESoon Won HONGWoojin JUNHong Yon CHOHan-Cheon KIMMyung Gu JUNGJiemin WONGHa-Il KIMChang-Hoon KIMHo-Geun YOON
ジャーナル フリー

2007 年 71 巻 11 号 p. 2712-2719


Histone acetylation depends on the activity of two enzyme families, histone acetyltransferase (HAT) and deacetylase (HDAC). In this study, we screened various plant extracts to find potent HAT inhibitors. Hot water extracts of allspice inhibited HAT activity, especially p300 and CBP (40% at 100 μg/ml). The mRNA levels of two androgen receptor (AR) regulated genes, PSA and TSC22, decreased with allspice treatment (100 μg/ml). Importantly, in IP western analysis, AR acetylation was dramatically decreased by allspice treatment.
Furthermore, chromatin immunoprecipitation indicated that the acetylation of histone H3 in the PSA and B2M promoter regions was also repressed. Finally, allspice treatment reduced the growth of human prostate cancer cells, LNCaP (50% growth inhibition at 200 μg/ml). Taken together, our data indicate that the potent HAT inhibitory activity of allspice reduced AR and histone acetylation and led to decreased transcription of AR target genes, resulting in inhibition of prostate cancer cell growth.



© 2007 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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