Immunoassay Using Microfluid Filters Constructed by Deep X-Ray Lithography

Katsuhiro MATSUI; Isao KAWAJI; Yuichi UTSUMI; Yoshiaki UKITA; Toshifumi ASANO; Masahiro TAKEO; Dai-ichiro KATO; Seiji NEGORO

doi:10.1271/bbb.70415

Analytical Chemistry Note

Immunoassay Using Microfluid Filters Constructed by Deep X-Ray Lithography

Katsuhiro MATSUI, Isao KAWAJI, Yuichi UTSUMI, Yoshiaki UKITA, Toshifumi ASANO, Masahiro TAKEO, Dai-ichiro KATO, Seiji NEGORO

Author information

Katsuhiro MATSUI
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
Isao KAWAJI
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
Yuichi UTSUMI
Laboratory of Advanced Science and Technology for Industry, University of Hyogo
Yoshiaki UKITA
Laboratory of Advanced Science and Technology for Industry, University of Hyogo
Toshifumi ASANO
Laboratory of Advanced Science and Technology for Industry, University of Hyogo
Masahiro TAKEO
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
Dai-ichiro KATO
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
Seiji NEGORO
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo

Keywords: vertical microfluidic operation, enzyme-linked immunosorbent assay, synchrotron radiation, microfluid filter, X-ray lithography

JOURNAL FREE ACCESS

2007 Volume 71 Issue 12 Pages 3098-3101

DOI https://doi.org/10.1271/bbb.70415

View "Advance Publication" version (December 7, 2007).

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Abstract

Microfluid filters were fabricated, which possessed 2,100 cylindrical through-bores (φ40 μm) in 200 μm-thickness polymethylmethacrylate (PMMA) sheets (φ3 mm), by deep X-ray lithography using synchrotron radiation. To evaluate the microfluid filters as a device for an immunoassay, we bound the goat anti-mouse immunogloblin G (IgG) antibody to the surface of the filters, and set the filters between reaction vessels stacked vertically in a microreactor. An enzyme-linked immunosorbent assay (ELISA) of mouse IgG using the goat anti-mouse IgG/horseradish-peroxidase (HRP) conjugate indicated that mouse IgG could be quantitatively detected in the range of 0–100 ng/ml, demonstrating the applicability of vertical microfluidic operation to the immunoassay.

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