Estimation of Luminal Mucin Content in Rats by Measurement of O-Linked Oligosaccharide Chains and Direct ELISA

Hiroki TANABE; Hiroyuki ITO; Kimio SUGIYAMA; Shuhachi KIRIYAMA; Tatsuya MORITA

doi:10.1271/bbb.60448

Food & Nutrition Science Notes

Estimation of Luminal Mucin Content in Rats by Measurement of O-Linked Oligosaccharide Chains and Direct ELISA

Hiroki TANABE, Hiroyuki ITO, Kimio SUGIYAMA, Shuhachi KIRIYAMA, Tatsuya MORITA

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Keywords: mucins, ELISA, O-linked oligosaccharide chains, rat

JOURNAL FREE ACCESS

2007 Volume 71 Issue 2 Pages 575-578

DOI https://doi.org/10.1271/bbb.60448

View "Advance Publication" version (February 7, 2007).

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Abstract

Changes in the small intestinal mucin contents in rats were evaluated by two methods, viz., a newly established ELISA and a method based on the measurement of O-linked oligosaccharide chains (OSC) as a mucin marker. Significant correlation was observed between the values of ELISA-derived mucins and OSC. The results confirm the usefulness of measurement of OSC as an alternative method for mucin determination.

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