Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
Expression of a Functional Sphingomyelinase of Pseudomonas sp. TK4 in Mammalian Cells
Yasuhiro HORIBATANoriyuki SUEYOSHIMakoto ITO
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2007 Volume 71 Issue 2 Pages 603-606

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Abstract

We report here the expression of a bacterial sphingomyelinase in mammalian cells as a functionally active form. A chimeric Pseudomonas sphingomyelinase fused with the lysosomal sorting motif of lysosomal acid phosphatase was sorted to lysosomes in mammalian cells. As expected, the chimeric SMase hydrolyzed sphingomyelin in vivo to produce ceramide, part of which was converted to glucosylceramide.

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© 2007 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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