A Method for Pmo25-Associated Kinase Assay in Fission Yeast: The Activity Is Dependent on Two GC Kinases Nak1 and Sid1

Kazunori KUME; Tetsuya GOSHIMA; Kohji MIYAHARA; Takashi TODA; Dai HIRATA

doi:10.1271/bbb.60574

Biochemistry & Molecular Biology Notes

A Method for Pmo25-Associated Kinase Assay in Fission Yeast: The Activity Is Dependent on Two GC Kinases Nak1 and Sid1

Kazunori KUME, Tetsuya GOSHIMA, Kohji MIYAHARA, Takashi TODA, Dai HIRATA

著者情報

キーワード: cell morphogenesis, kinase assay, MO25, Nak1, Sid1

ジャーナルフリー

2007 年 71 巻 2 号 p. 615-617

DOI https://doi.org/10.1271/bbb.60574

詳細

抄録

In fission yeast, the conserved proteins, MO25/Pmo25, GC kinase/Nak1, Furry/Mor2, NDR kinase/Orb6, and Mob2, constitute the morphogenesis Orb6 network (MOR). Previously we showed that Pmo25 functions as an upstream component of MOR and that it plays a connecting role between the septation initiation network (SIN) and MOR. Here we establish a Pmo25-associated kinase assay and show that the activity is dependent on Nak1/MOR and Sid1/SIN.

責任著者(Corresponding author)

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