Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Communications
Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants
Tsuyoshi NAKAGAWATakamasa SUZUKISatoko MURATAShinya NAKAMURATakeshi HINOKenichiro MAEORyo TABATATsutae KAWAIKatsunori TANAKAYasuo NIWAYuichiro WATANABEKenzo NAKAMURATetsuya KIMURASumie ISHIGURO
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2007 Volume 71 Issue 8 Pages 2095-2100

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Abstract

We made a series of improved Gateway binary vectors (pGWBs) for plant transformation. Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6×His, FLAG, 3×HA, 4×Myc, 10×Myc, GST, T7, and TAP, were employed. Some vectors carry the 2×35S-Ω promoter for higher-level expression. The kanamycin- and hygromycin-resistant markers are independently available for each of the 43 types of vectors, thus an additional transformation of once-transformed plants can be carried out easily. Their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing. Improved pGWBs should be a powerful tool for transgenic research in plants.

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© 2007 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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