Differential Chain-Length Specificities of Two Isoamylase-Type Starch-Debranching Enzymes from Developing Seeds of Kidney Bean

Yoshinori TAKASHIMA; Takeshi SENOURA; Takayuki YOSHIZAKI; Shigeki HAMADA; Hiroyuki ITO; Hirokazu MATSUI

doi:10.1271/bbb.70215

Biochemistry & Molecular Biology Notes

Differential Chain-Length Specificities of Two Isoamylase-Type Starch-Debranching Enzymes from Developing Seeds of Kidney Bean

Yoshinori TAKASHIMA, Takeshi SENOURA, Takayuki YOSHIZAKI, Shigeki HAMADA, Hiroyuki ITO, Hirokazu MATSUI

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Keywords: kidney bean (Phaseolus vulgaris L.), isoamylase, starch-debranching enzyme, amylopectin

JOURNAL FREE ACCESS

2007 Volume 71 Issue 9 Pages 2308-2312

DOI https://doi.org/10.1271/bbb.70215

View "Advance Publication" version (September 7, 2007).

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Abstract

Plant isoamylase-type starch-debranching enzymes (ISAs) hydrolyze α-1,6-linkages in α-1,4/α-1,6-linked polyglucans. Two ISAs, designated PvISA1/2 and PvISA3, were purified from developing seeds of kidney bean by ammonium sulfate fractionation and several column chromatographic procedures. The enzymes displayed different substrate specificities for polyglucans: PvISA1/2 showed broad chain-length specificities, whereas PvISA3 liberated specific chains with a DP of 2 to 4.

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