Expression and DNA Binding Activity of the Tomato Transcription Factor RIN (Ripening Inhibitor)

Hong-Liang ZHU; Ben-Zhong ZHU; Ying-Cong LI; Yi SHAO; Xiao-Guang WANG; Yuan-Hong XIE; An-Jun CHEN; Jun-Jie LUO; Xiao-Yan JIA; Yun-Bo LUO

doi:10.1271/bbb.70577

Biochemistry & Molecular Biology Notes

Expression and DNA Binding Activity of the Tomato Transcription Factor RIN (Ripening Inhibitor)

Hong-Liang ZHU, Ben-Zhong ZHU, Ying-Cong LI, Yi SHAO, Xiao-Guang WANG, Yuan-Hong XIE, An-Jun CHEN, Jun-Jie LUO, Xiao-Yan JIA, Yun-Bo LUO

著者情報

Hong-Liang ZHU
Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University
Ben-Zhong ZHU
Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University
Ying-Cong LI
Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University
Yi SHAO
Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University
Xiao-Guang WANG
Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University
Yuan-Hong XIE
Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University
An-Jun CHEN
Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University
Jun-Jie LUO
Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University
Xiao-Yan JIA
Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University
Yun-Bo LUO
Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University

キーワード: binding activity, electrophoretic mobility shift assay (EMSA), Mcm1, Agamous, Deficiens, Srf (MADS)-box, ripening inhibitor, tomato

ジャーナルフリー

2008 年 72 巻 1 号 p. 250-252

DOI https://doi.org/10.1271/bbb.70577

View "Advance Publication" version (January 7, 2008).

詳細

抄録

Recently, we have found that the accumulation of ripening inhibitor (RIN) protein increased gradually during tomato fruit ripening. Here, the recombinant protein was expressed in Escherichia coli and affinity-purified. The DNA binding activity of renatured RIN protein was tested by electrophoretic mobility shift assay. The results indicated that an optimal expression and purification system was suitable for obtaining active RIN with DNA binding activity.

責任著者(Corresponding author)

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