Selection of an Effective Red-Pigment Producing Monascus pilosus by Efficient Transformation with Aurintricarboxylic Acid

Yi-Pei CHEN; I-Ching CHEN; Ing-Er HWANG; Gwo-Fang YUAN; Li-Ling LIAW; Ching-Ping TSENG

doi:10.1271/bbb.80342

Biochemistry & Molecular Biology Notes

Selection of an Effective Red-Pigment Producing Monascus pilosus by Efficient Transformation with Aurintricarboxylic Acid

Yi-Pei CHEN, I-Ching CHEN, Ing-Er HWANG, Gwo-Fang YUAN, Li-Ling LIAW, Ching-Ping TSENG

著者情報

キーワード: Monascus pilosus, restriction-enzyme-mediated integration (REMI), aurintricarboxylic acid (ATA)

ジャーナルフリー

2008 年 72 巻 11 号 p. 3021-3024

DOI https://doi.org/10.1271/bbb.80342

View "Advance Publication" version (November 7, 2008).

詳細

抄録

The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction-enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation.

責任著者(Corresponding author)

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