Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
Construction of a Binary Vector for Transformation of Arabidopsis thaliana with a New Selection Marker
Nozomu KOIZUMIYuji IWATA
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JOURNAL FREE ACCESS

2008 Volume 72 Issue 11 Pages 3041-3043

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Abstract

A new binary vector, pZT4B, containing the UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as a selection marker, was constructed. The green fluorescent protein (GFP) gene was inserted into pZT4B, and the resulting plasmid was used in the transformation of Arabidopsis. All of six independent transformants obtained after selection with 0.3 mg/l tunicamycin contained the transgene and showed GFP fluorescence.

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© 2008 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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