Improvement of <small>L</small>-Lactate Production by CYB2 Gene Disruption in a Recombinant Saccharomyces cerevisiae Strain under Low pH Condition

Aki OOKUBO; Takashi HIRASAWA; Katsunori YOSHIKAWA; Keisuke NAGAHISA; Chikara FURUSAWA; Hiroshi SHIMIZU

doi:10.1271/bbb.80493

Microbiology & Fermentation Technology Notes

Improvement of L-Lactate Production by CYB2 Gene Disruption in a Recombinant Saccharomyces cerevisiae Strain under Low pH Condition

Aki OOKUBO, Takashi HIRASAWA, Katsunori YOSHIKAWA, Keisuke NAGAHISA, Chikara FURUSAWA, Hiroshi SHIMIZU

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Keywords: Saccharomyces cerevisiae, L-lactate, DNA microarray, CYB2, pH

JOURNAL FREE ACCESS

2008 Volume 72 Issue 11 Pages 3063-3066

DOI https://doi.org/10.1271/bbb.80493

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Abstract

Using a DNA microarray, we found that expression of the genes related to lactate metabolism was upregulated in a lactate-producing recombinant Saccharomyces cerevisiae strain. Disruption of the CYB2 gene encoding L-lactate dehydrogenase improved the L-lactate production by S. cerevisiae under low pH condition.

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