Functional Identification of a Rice ent-Kaurene Oxidase, OsKO2, Using the Pichia pastoris Expression System

Kwang-Wook KO; Fengqiu LIN; Takumi KATSUMATA; Yoshinori SUGAI; Sho MIYAZAKI; Hiroshi KAWAIDE; Kazunori OKADA; Hideaki NOJIRI; Hisakazu YAMANE

doi:10.1271/bbb.80427

Biochemistry & Molecular Biology Notes

Functional Identification of a Rice ent-Kaurene Oxidase, OsKO2, Using the Pichia pastoris Expression System

Kwang-Wook KO, Fengqiu LIN, Takumi KATSUMATA, Yoshinori SUGAI, Sho MIYAZAKI, Hiroshi KAWAIDE, Kazunori OKADA, Hideaki NOJIRI, Hisakazu YAMANE

Author information

Kwang-Wook KO
Biotechnology Research Center, The University of Tokyo
Fengqiu LIN
Biotechnology Research Center, The University of Tokyo
Takumi KATSUMATA
Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology (TUAT)
Yoshinori SUGAI
Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology (TUAT)
Sho MIYAZAKI
Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology (TUAT)
Hiroshi KAWAIDE
Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology (TUAT)
Kazunori OKADA
Biotechnology Research Center, The University of Tokyo
Hideaki NOJIRI
Biotechnology Research Center, The University of Tokyo
Hisakazu YAMANE
Biotechnology Research Center, The University of Tokyo

Keywords: ent-kaurene oxidase, cytochrome P450 monooxygenase (P450), rice

JOURNAL FREE ACCESS

2008 Volume 72 Issue 12 Pages 3285-3288

DOI https://doi.org/10.1271/bbb.80427

View "Advance Publication" version (December 7, 2008).

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Abstract

Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.

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