Partition Coefficients of Polyphenols for Phosphatidylcholine Investigated by HPLC with an Immobilized Artificial Membrane Column

Yoshinori UEKUSA; Yuko TAKESHITA; Takeshi ISHII; Tsutomu NAKAYAMA

doi:10.1271/bbb.80435

Food & Nutrition Science Notes

Partition Coefficients of Polyphenols for Phosphatidylcholine Investigated by HPLC with an Immobilized Artificial Membrane Column

Yoshinori UEKUSA, Yuko TAKESHITA, Takeshi ISHII, Tsutomu NAKAYAMA

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Keywords: polyphenol, immobilized artificial membrane (IAM), HPLC, lipid membranes, IAM partition coefficient (K_IAM)

JOURNAL FREE ACCESS

2008 Volume 72 Issue 12 Pages 3289-3292

DOI https://doi.org/10.1271/bbb.80435

View "Advance Publication" version (December 7, 2008).

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Abstract

The biological activity of polyphenols in vitro reflects their affinity for cell membranes and the amount of cellular incorporation. The interaction of polyphenols with phosphatidylcholine was investigated by HPLC with an immobilized artificial membrane (IAM) column. The IAM partition coefficients (K_IAM) of the polyphenols, calculated by retention times, correlated well with the amounts of polyphenols incorporation into the liposomes.

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