Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Communication
Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research
Tsuyoshi NAKAGAWAShinya NAKAMURAKatsunori TANAKAMakoto KAWAMUKAITakamasa SUZUKIKenzo NAKAMURATetsuya KIMURASumie ISHIGURO
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2008 Volume 72 Issue 2 Pages 624-629

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Abstract

We developed a new series of Gateway binary vectors, R4pGWBs, that are plant transformation vectors designed for one-step construction of chimeric genes between any promoter and any cDNA. The structure of R4pGWBs is almost the same as the promoterless type of improved pGWBs (ImpGWBs), except that the attR1 site is replaced with attR4, which enables tripartite recombination of these vectors with promoter- and cDNA-entry clones. While ImpGWBs are suitable for promoter analysis and constitutive expression of cDNAs in higher plants, R4pGWBs have a great advantage in expressing a cDNA under the regulation of desired promoters.

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© 2008 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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